FM1-43

Catalog No.GC67896
FM1-43 is a water-soluble styrene dye with strong lipophilicity.

Description
FM1 43 is a water-soluble styrene dye with strong lipophilicity. FM 1-43 membrane probe is a reagent that can specifically bind to cell membranes and inner membrane organelles to generate fluorescence. FM1-43 is widely used as endocytosis and excision membrane structure markers, and can be used to identify actively firing neurons and research Mechanisms of activity-dependent vesicle cycling[1]. In actively releasing neurotransmitters, the FM1-43 dye is internalized into recycled synaptic vesicles, causing the nerve terminals to become brightly stained. Non-specific staining of cell surface membranes from FM1-43 can be simply washed off before observation[2]. FM1-43 is nontoxic to cells, barely fluoresces in aqueous media, but fluoresces strongly after insertion into the outer leaflet of the cell membrane. FM1-43 also reduces the ototoxic effects of the aminoglycoside antibiotic neomycin sulfate[3].

References:
[1]. Audrey C Brumback, et al. Using FM1-43 to study neuropeptide granule dynamics and exocytosis.2004 Aug;33(4):287-94. doi: 10.1016/j.ymeth.2004.01.002.
[2]. J J Renger,C Egles, G Liu. A developmental switch in neurotransmitter flux enhances synaptic efficacy by affecting AMPA receptor activation.2001 Feb;29(2):469-84. doi: 10.1016/s0896-6273(01)00219-7.
[3]. J E Gale, et al. FM1-43 dye behaves as a permeant blocker of the hair-cell mechanotransducer channel. J Neurosci. 2001 Sep 15;21(18):7013-25.

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